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名称：防风指针成分之制备与品质管制
主持人：杨玲玲
防风(Saposhnikovia
divaricata (Turcz.) Schischk.)属于伞形(Umbelliferae)的植物，最早收录于神农本草经，列为草部上品，其学名及市场代用品极为复杂；而许多常用的中药方剂如：荆防败毒散、消风散、防风通圣散、玉屏风散、独活寄生汤....等都含有防风，为确保国人用药安全及中药制剂疗效之可靠性，应对防风之原料药材进行成分分析，建立其药材指针成分及定量分析之模式。
本计画由防风中抽取纯化特有之镇痛、降血压之活性成分cimifugin作为此药材之指针成分，并建立防风药材中cimifugin的高效率液相层析定量分析模式；其分析条件为CH3CN-H2O(18:82)为移动相，流速为1.0ml/min，以LiChrospher
100 RP-18e (4 mm i.d.(250 mm)为分析管柱，以波长220nm检测，在40(C下进行分析。防风中cimifugin的最佳抽取条件为以二十倍量70%甲醇水溶液超音波震荡四十分钟抽取可得最高的产率；并以此定量模式分析市售之防风药材中cimifugin指针成分含量，以作为未来卫生署制定中药制剂品质管制定量之参考。
关键词：防风，cimifugin，指针成分，品质管制，市售品
Code
Number:CCMP87-RD-029
Title:The
Marker Standard Preparation of Saposhnikoviae Radix and Quality
Control
Orgazination:Graduate
Institute of Pharmacognosy Science Taipei Medical College
Auther:Ling-Ling
Yang
Cimifugin,
one of the analgesic and anti-hypertension principle component of
Fang-Feng, was isolated from the root of Saposhnikovia divaricata.
Reversed-phase high-performance liquid chromatography was employed
to determine the contents of cimifugin in Fang-Feng. The separation
was performed on a LiChrospher 100 RP-18e column (4 mm I.D.×250
mm) by isocretic elution with acetonitrile-water (v/v) (18:82) as
the mobile phase at a flow-rate of 1.0 ml/min, the temperature keep
at 40℃ and with detection at 220 nm. Methylparaben
was used as the internal standard and a regression equation revealed
linear relationship between the peak-area ratio (marker substance/internal
standard) and concentration. We found that the decoction from Fang-Feng
with 20 times of 70% aqueous methanol in 40 minutes of sonication
given the best yield of cimifugin. This HPLC method can rapidly
and simply quantify the cimifugin present in Fang-Feng and can be
used for quality control of commercial crude drug of Fang-Feng.
Keywords:Saposhnikovia
divaricata,cimifugin, marker substance, quality control, commercial
crude drug
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